Journal: PLoS ONE
Article Title: Effects of Simulated Microgravity on Human Umbilical Vein Endothelial Cell Angiogenesis and Role of the PI3K-Akt-eNOS Signal Pathway
doi: 10.1371/journal.pone.0040365
Figure Lengend Snippet: A. Tube-like structures of the Cont group, B. Tube-like structures of the Clino group, C. Tube-like structures of the Clino + L-NAME group. Shown are representative pictures of HUVEC-C plated on Matrigel for 20 h. D. Quantified tube formation as described in . Simulated microgravity significantly promoted the tube formation of HUVEC-C. Co-incubation with L-NAME, the specific inhibitor of eNOS, dramatically suppressed the HUVEC-C tube formation (n = 3, mean ± S.D.). ** P <0.01 versus control group; ## P <0.01 versus clinorotation group.
Article Snippet: Matrigel™ matrix (basement membrane) was obtained from BD Biosciences (Oxford, U.K.). eNOS inhibitor, N-nitro-L-arginine methylester hydrochloride (L-NAME) was obtained from Sigma.
Techniques: Incubation